Geometric Support for Dark Matter by an Unaligned Einstein Ring in Abell 3827

The non-detection of dark matter (DM) particles in increasingly stringent laboratory searches has encouraged alternative gravity theories where gravity is sourced only from visible matter. Here, we consider whether such theories can pass a two-dimensional test posed by gravitational lensing -- to reproduce a particularly detailed Einstein ring in the core of the galaxy cluster Abell 3827. We find that when we require the lensing mass distribution to strictly follow the shape (ellipticity and position angle) of the light distribution of cluster member galaxies, intracluster stars, and the X-ray emitting intracluster medium, we cannot reproduce the Einstein ring, despite allowing the mass-to-light ratios of these visible components to freely vary with radius to mimic alternative gravity theories. Alternatively, we show that the detailed features of the Einstein ring are accurately reproduced by allowing a smooth, freely oriented DM halo in the lens model, with relatively small contributions from the visible components at a level consistent with their observed brightnesses. This dominant DM component is constrained to have the same orientation as the light from the intracluster stars, indicating that the intracluster stars trace the gravitational potential of this component. The Einstein ring of Abell 3827 therefore presents a new challenge for alternative gravity theories: not only must such theories find agreement between the total lensing mass and visible mass, but they must also find agreement between the projected sky distribution of the lensing mass and that of the visible matter, a more stringent test than has hitherto been posed by lensing data.Comment: 20 pages, 7 figures, 8 tables. Accepted for publication in Ap

The Cosmic Ultraviolet Baryon Survey (CUBS) I. Overview and the diverse environments of Lyman limit systems at z<1

We present initial results from the Cosmic Ultraviolet Baryon Survey (CUBS). CUBS is designed to map diffuse baryonic structures at redshift z<~1 using absorption-line spectroscopy of 15 UV-bright QSOs with matching deep galaxy survey data. CUBS QSOs are selected based on their NUV brightness to avoid biases against the presence of intervening Lyman Limit Systems (LLSs) at zabs~ 17.2 over a total redshift survey pathlength of dz=9.3, and a number density of n(z)=0.43 (-0.18, +0.26). Considering all absorbers with log N(HI)/cm^-2 > 16.5 leads to n(z)=1.08 (-0.25, +0.31) at z<1. All LLSs exhibit a multi-component structure and associated metal transitions from multiple ionization states such as CII, CIII, MgII, SiII, SiIII, and OVI absorption. Differential chemical enrichment levels as well as ionization states are directly observed across individual components in three LLSs. We present deep galaxy survey data obtained using the VLT-MUSE integral field spectrograph and the Magellan Telescopes, reaching sensitivities necessary for detecting galaxies fainter than 0.1L* at d<~300 physical kpc (pkpc) in all five fields. A diverse range of galaxy properties is seen around these LLSs, from a low-mass dwarf galaxy pair, a co-rotating gaseous halo/disk, a star-forming galaxy, a massive quiescent galaxy, to a galaxy group. The closest galaxies have projected distances ranging from d=15 to 72 pkpc and intrinsic luminosities from ~0.01L* to ~3L*. Our study shows that LLSs originate in a variety of galaxy environments and trace gaseous structures with a broad range of metallicities.Comment: 26 pages, 14 figures, MNRAS in pres

The first comprehensive study of a giant nebula around a radio-quiet quasar in the z<1z < 1 Universe

We present the first comprehensive study of a giant, $\approx \! \! 70$ kpc-scale nebula around a radio-quiet quasar at $z<1$. The analysis is based on deep integral field spectroscopy with MUSE of the field of HE$\,$0238$-$1904, a luminous quasar at $z=0.6282$. The nebula emits strongly in $\mathrm{[O \, II]}$, $\rm H \beta$, and $\mathrm{[O \, III]}$, and the quasar resides in an unusually overdense environment for a radio-quiet system. The environment likely consists of two groups which may be merging, and in total have an estimated dynamical mass of $M_{\rm dyn}\approx 4\times 10^{13}$ to $10^{14}\ {\rm M_\odot}$. The nebula exhibits largely quiescent kinematics and irregular morphology. The nebula may arise primarily through interaction-related stripping of circumgalactic and interstellar medium (CGM/ISM) of group members, with some potential contributions from quasar outflows. The simultaneous presence of the giant nebula and a radio-quiet quasar in a rich environment suggests a correlation between such circum-quasar nebulae and environmental effects. This possibility can be tested with larger samples. The upper limits on the electron number density implied by the$\mathrm{[O \, II]}$doublet ratio range from$\log(n_{\rm e, \, [O \, II]} / \mathrm{cm^{-3}}) < 1.2$to$2.8$. However, assuming a constant quasar luminosity and negligible projection effects, the densities implied from the measured line ratios between different ions (e.g.,$\mathrm{[O\,II]}$,$\mathrm{[O\,III]}$, and$\mathrm{[Ne\,V]}$) and photoionization simulations are often$10{-}400$times larger. This large discrepancy can be explained by quasar variability on a timescale of$\approx 10^4{-}10^5$ years.Comment: 19 pages, 9 figures, 3 tables; Submitted to MNRA

The Cosmic Ultraviolet Baryon Survey (CUBS) V: On the Thermodynamic Properties of the Cool Circumgalactic Medium at z<1z < 1

This paper presents a systematic study of the photoionization and thermodynamic properties of the cool circumgalactic medium (CGM) as traced by rest-frame ultraviolet absorption lines around 26 galaxies at redshift $z\lesssim1$. The study utilizes both high-quality far-ultraviolet and optical spectra of background QSOs and deep galaxy redshift surveys to characterize the gas density, temperature, and pressure of individual absorbing components and to resolve their internal non-thermal motions. The derived gas density spans more than three decades, from $\log (n_{\rm H}/{\rm cm^{-3}}) \approx -4$ to $-1$, while the temperature of the gas is confined in a narrow range of $\log (T/{\rm K})\approx 4.3\pm 0.3$. In addition, a weak anti-correlation between gas density and temperature is observed, consistent with the expectation of the gas being in photoionization equilibrium. Furthermore, decomposing the observed line widths into thermal and non-thermal contributions reveals that more than 30% of the components at $z\lesssim 1$ exhibit line widths driven by non-thermal motions, in comparison to $<20$% found at $z\approx 2$-3. Attributing the observed non-thermal line widths to intra-clump turbulence, we find that massive quenched galaxies on average exhibit higher non-thermal broadening/turbulent energy in their CGM compared to star-forming galaxies at $z\lesssim 1$. Finally, strong absorption features from multiple ions covering a wide range of ionization energy (e.g., from Mg II to O IV) can be present simultaneously in a single absorption system with kinematically aligned component structure, but the inferred pressure in different phases may differ by a factor of $\approx 10$.Comment: 16 pages, 4 figures, 1 table, accepted for publication in MNRA

OXPHOS Supercomplexes as a Hallmark of the Mitochondrial Phenotype of Adipogenic Differentiated Human MSCs

Mitochondria are essential organelles with multiple functions, especially in energy metabolism. Recently, an increasing number of data has highlighted the role of mitochondria for cellular differentiation processes. Metabolic differences between stem cells and mature derivatives require an adaptation of mitochondrial function during differentiation. In this study we investigated alterations of the mitochondrial phenotype of human mesenchymal stem cells undergoing adipogenic differentiation. Maturation of adipocytes is accompanied by mitochondrial biogenesis and an increase of oxidative metabolism. Adaptation of the mt phenotype during differentiation is reflected by changes in the distribution of the mitochondrial network as well as marked alterations of gene expression and organization of the oxidative phosphorylation system (OXPHOS). Distinct differences in the supramolecular organization forms of cytochrome c oxidase (COX) were detected using 2D blue native (BN)-PAGE analysis. Most remarkably we observed a significant increase in the abundance of OXPHOS supercomplexes in mitochondria, emphasizing the change of the mitochondrial phenotype during adipogenic differentiation

Kinetoplastid Phylogenomics Reveals the Evolutionary Innovations Associated with the Origins of Parasitism

The evolution of parasitism is a recurrent event in the history of life and a core problem in evolutionary biology. Trypanosomatids are important parasites and include the human pathogens Trypanosoma brucei, Trypanosoma cruzi, and Leishmania spp., which in humans cause African trypanosomiasis, Chagas disease, and leishmaniasis, respectively. Genome comparison between trypanosomatids reveals that these parasites have evolved specialized cell-surface protein families, overlaid on a well-conserved cell template. Understanding how these features evolved and which ones are specifically associated with parasitism requires comparison with related non-parasites. We have produced genome sequences for Bodo saltans, the closest known non-parasitic relative of trypanosomatids, and a second bodonid, Trypanoplasma borreli. Here we show how genomic reduction and innovation contributed to the character of trypanosomatid genomes. We show that gene loss has “streamlined” trypanosomatid genomes, particularly with respect to macromolecular degradation and ion transport, but consistent with a widespread loss of functional redundancy, while adaptive radiations of gene families involved in membrane function provide the principal innovations in trypanosomatid evolution. Gene gain and loss continued during trypanosomatid diversification, resulting in the asymmetric assortment of ancestral characters such as peptidases between Trypanosoma and Leishmania, genomic differences that were subsequently amplified by lineage-specific innovations after divergence. Finally, we show how species-specific, cell-surface gene families (DGF-1 and PSA) with no apparent structural similarity are independent derivations of a common ancestral form, which we call “bodonin.” This new evidence defines the parasitic innovations of trypanosomatid genomes, revealing how a free-living phagotroph became adapted to exploiting hostile host environments

Interaction of the Deubiquitinating Enzyme Ubp2 and the E3 Ligase Rsp5 Is Required for Transporter/Receptor Sorting in the Multivesicular Body Pathway

Protein ubiquitination is essential for many events linked to intracellular protein trafficking. We sought to elucidate the possible involvement of the S. cerevisiae deubiquitinating enzyme Ubp2 in transporter and receptor trafficking after we (this study) and others established that affinity purified Ubp2 interacts stably with the E3 ubiquitin ligase Rsp5 and the (ubiquitin associated) UBA domain containing protein Rup1. UBP2 interacts genetically with RSP5, while Rup1 facilitates the tethering of Ubp2 to Rsp5 via a PPPSY motif. Using the uracil permease Fur4 as a model reporter system, we establish a role for Ubp2 in membrane protein turnover. Similar to hypomorphic rsp5 alleles, cells deleted for UBP2 exhibited a temporal stabilization of Fur4 at the plasma membrane, indicative of perturbed protein trafficking. This defect was ubiquitin dependent, as a Fur4 N-terminal ubiquitin fusion construct bypassed the block and restored sorting in the mutant. Moreover, the defect was absent in conditions where recycling was absent, implicating Ubp2 in sorting at the multivesicular body. Taken together, our data suggest a previously overlooked role for Ubp2 as a positive regulator of Rsp5-mediated membrane protein trafficking subsequent to endocytosis

Pathogenic TNF-α drives peripheral nerve inflammation in an Aire-deficient model of autoimmunity

Immune cells infiltrate the peripheral nervous system (PNS) after injury and with autoimmunity, but their net effect is divergent. After injury, immune cells are reparative, while in inflammatory neuropathies (e.g., Guillain Barré Syndrome and chronic inflammatory demyelinating polyneuropathy), immune cells are proinflammatory and promote autoimmune demyelination. An understanding of immune cell phenotypes that distinguish these conditions may, therefore, reveal new therapeutic targets for switching immune cells from an inflammatory role to a reparative state. In an autoimmune regulator (Aire)-deficient mouse model of inflammatory neuropathy, we used single-cell RNA sequencing of sciatic nerves to discover a transcriptionally heterogeneous cellular landscape, including multiple myeloid, innate lymphoid, and lymphoid cell types. Analysis of cell-cell ligand-receptor interactions uncovered a macrophage-mediated tumor necrosis factor-α (TNF-α) signaling axis that is induced by interferon-γ and required for initiation of autoimmune demyelination. Developmental trajectory visualization suggested that TNF-α signaling is associated with metabolic reprogramming of macrophages and polarization of macrophages from a reparative state in injury to a pathogenic, inflammatory state in autoimmunity. Autocrine TNF-α signaling induced macrophage expression of multiple genes (Clec4e, Marcksl1, Cxcl1, and Cxcl10) important in immune cell activation and recruitment. Genetic and antibody-based blockade of TNF-α/TNF-α signaling ameliorated clinical neuropathy, peripheral nerve infiltration, and demyelination, which provides preclinical evidence that the TNF-α axis may be effectively targeted to resolve inflammatory neuropathies